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Determination of substances in phytopharmaceuticals
Ivanishchanka, Iryna ; Řezáčová, Veronika (referee) ; Vávrová, Milada (advisor)
In the bachelor thesis, herbal teas, the main components of which are St. John's Wort and Lemon balm, were analyzed. The physiological properties, the chemical composition and the influence on human health were monitored. Thesis deals method of isolation and identification of essentials oils from out of selected teas to calm the nervous system. For isolation of essentials oils there are used methods solvent extraction, solid phase microextraction (SPME) and steam distillation, for identification there is gas chromatography (GC/MS). Isolation methods for obtaining essential oils from plant matrices were assessed and the most appropriate isolation techniques was determined by solid phase microextraction (SPME)
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Phytochemical studies of potential phytopharmaceuticals using separation methods
Dulendarova, Elizabeta Hristiyanova ; Jenčo, Jaroslav (advisor) ; Křoustková, Jana (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department: Department of Pharmacognosy and Pharmaceutical Botany Candidate: Elizabeta Hristiyanova Dulendarova Supervisor: RNDr. Jaroslav Jenčo, Ph.D. Title of dissertation thesis: Phytochemical Studies of Potential Phytopharmaceuticals using Separation Methods The main aim of this diploma thesis was the identification of main alkaloidal components from the fraction of Papaver rhoeas L. (family Papaveraceae) alkaloidal extract - fraction PR-12-23. The introduction to the theoretical part summarizes alkaloids found in Papaver rhoeas L. and describes the separation methods used in the isolation process at the department of Phamacognosy and Pharmaceutical botany. Two main compounds within the extract were identified and separated based on HPTLC, HPLC-MS, and GC-MS chromatographic separation methods. For the subsequent fractions, the HPLC method development, optimization, and scaling up from analytical to preparative scale was done. After identification with the help of analytical HPLC and GC, the compounds were separated into fractions, isolated using preparative HPLC, and identified using NMR. The purified isolates were tested for anti-butyrylcholinesterase and cytotoxic biological activity, linked to AD and cancer, respectively. The first...
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Determination of substances in phytopharmaceuticals
Ivanishchanka, Iryna ; Řezáčová, Veronika (referee) ; Vávrová, Milada (advisor)
In the bachelor thesis, herbal teas, the main components of which are St. John's Wort and Lemon balm, were analyzed. The physiological properties, the chemical composition and the influence on human health were monitored. Thesis deals method of isolation and identification of essentials oils from out of selected teas to calm the nervous system. For isolation of essentials oils there are used methods solvent extraction, solid phase microextraction (SPME) and steam distillation, for identification there is gas chromatography (GC/MS). Isolation methods for obtaining essential oils from plant matrices were assessed and the most appropriate isolation techniques was determined by solid phase microextraction (SPME)
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